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. 2018 Jan 25;8:1975. doi: 10.3389/fimmu.2017.01975

Figure 1.

Figure 1

Cathepsin G (CG) transcript is detected in B-ALL patient samples and cell lines. (A) According to Cancer Cell Line Encyclopedia (CCLE), ALL cell lines express CG transcript at higher levels compared with other malignancies. High-expressing acute myeloid leukemia (AML) cell lines are shown for comparison. (B) CG mRNA expression is not ubiquitous to all ALL. CG levels are lower in B-ALL and T-ALL cell lines compared with U937 and HL60 AML cell lines, but are higher compared with solid tumors; MDA-MB-231 (breast cancer), MCF7 (breast cancer), SW620 (colorectal adenocarcinoma), OVCAR-3 (ovarian adenocarcinoma), and MIA PaCa-2 (pancreatic carcinoma). (C) The Cancer Genome Atlas shows higher CG expression at the transcript level in B-ALL compared to normal B cells. Additionally, mRNA expression is higher in relapsed B-ALL compared with B-ALL at time of diagnosis. T-ALL has the highest CG expression at the transcript level. (D) RT-PCR of patient B-ALL shows prominent CG expression in three B-ALL patient samples (UPN3, UPN5, and UPN7; Table 1). AML cell line U937 and breast cancer cell line MDA-MB-231 were used as positive and negative controls, respectively. Actin was used as a loading control. The white boxes outlining the MDA-MB-231 bands indicate that they were transposed from a different region of the same gel.