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. 2018 Jan 25;8:1975. doi: 10.3389/fimmu.2017.01975

Figure 3.

Figure 3

PMN-associated Cathepsin G (CG) is taken up by B-ALL cell lines. (A) Flow cytometry detected intracellular CG in B-ALL cell lines after coculture with whole PMN at a ratio of 3:1 for 12 h. Cells were intracellularly stained with anti-CG antibody. PMN and peripheral blood mononuclear cells (PBMC) were used as positive and negative staining controls, respectively. *P < 0.05; **P < 0.01; ***P < 0.001. (B) RS4;11 cells were cocultured with whole PMN or PBMC at a ratio of 3:1 for 2, 4, 12, and 24 h and analyzed by flow cytometry for intracellular uptake of CG using anti-CG antibody. Cells cultured in the presence or absence of PBMC were used as negative controls. PMN and PBMC were used as positive and negative staining controls, respectively. Flow cytometry indicated that uptake of extracellular CG occurs in HMY2.CIR, Nalm6, and RS4;11 cell lines. Graphs display the mean ± SEM fold increase in median fluorescence intensity (MFI). Data represent triplicate wells from four independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001.