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. 2018 Jan 26;11:571–585. doi: 10.2147/OTT.S152611

Figure 4.

Figure 4

Direct regulation of PTEN expression by miR-106b-5p.

Notes: Schematic diagram of the dual luciferase miRNA target reporter vector (A), the wild type and mutant PTEN 3′-UTR sequences are shown with the miR-106b-5p sequence. (B) Western blot analysis of PTEN protein level in HCCLM3 cells transfected with miR-106b-5p inhibitors or NC (left), and PLC/5 cells transfected with miR-106b-5p mimics or NC (right). (C) qRT-PCR analysis of PTEN mRNA levels in five HCC cancer cell lines with different metastatic potentials and a normal liver cell line. (D) Luciferase assays in HEK293T cells with wild-type or mutant PTEN 3′-UTR vectors and miR-106b-5p mimic or NC. *P<0.05.

Abbreviations: PTEN, phosphatase and tensin homolog; HCC, hepatocellular carcinoma; WT, wild type; NC, negative control; qRT-PCR, quantitative real-time polymerase chain reaction.