Figure 5.

OVA-specific IgG antibody responses in BALB/c mice subcutaneously inoculated with various vaccine formulations.

Notes: Ninety mice were randomly divided into six groups with 15 mice in each, were injected subcutaneously twice, at day 0 and day 14, with 50 µg OVA in 200 µL formulation of each vaccine as follows: OVA-PHYP (50 µg PHY encapsulated) and OVA-PHY (50 µg PHY solution physical blended with OVA), OVA-PLA, OVA-FIA, free OVA (OVA dissolved in deionized water), and normal saline. Mice were sacrificed on days 7, 14, 21, 28, and 35, and sera were collected. (A) OD (450 nm) values of IgG in the serum at the indicated time points after the second immunization. (B) IgG2a/IgG1 ratios in the serum of immunized mice (serum diluted 1:5). Data are expressed as mean ± SEM (n=3). ^#p<0.05, and ^###p<0.001 vs OVA-PHY group, while *p<0.05, **p<0.01, and ***p<0.001 vs the OVA-PLA group.

Abbreviations: OVA, ovalbumin; OVA-PLA, OVA-encapsulated PLA; OVA-PHY, physical mixture of OVA and PHY; OVA-PHYP, OVA-loaded PHYP; PHY, pachyman; PHYP, PHY-encapsulated PLA; PLA, poly(D,L-lactic acid); SEM, standard error of the mean; IgG, immunoglobulin G.