Figure 7.

Results of lymphocyte proliferation assay.

Notes: Fresh separated splenocytes stimulated with OVA, LPS (as B-cell mitogen), or PHA (as T-cell mitogen) were seeded in triplicate (100 µL/well), each group in 96-well plates, and incubated in a humid atmosphere with 5% CO₂ for 72 h at 37°C. Lymphocyte proliferation assays after stimulation (A) and on synergistic stimulation with LPS (B) or PHA (C) were measured by MTT assay. Data represent mean ± SEM of triplicate experiments. ^#p<0.05, ^##p<0.01 and ^###p<0.001 vs OVA-PHY group, while *p<0.05, **p<0.01 and ***p<0.001 vs the OVA-PLA group.

Abbreviations: LPS, lipopolysaccharide; OVA, ovalbumin; OVA-PLA, OVA-encapsulated PLA; PHA, phytohemagglutinin; OVA-PHY, physical mixture of OVA and PHY; OVA-PHYP, OVA-loaded PHYP; PHY, pachyman; PHYP, PHY-encapsulated PLA; PLA, poly(D,L-lactic acid); SEM, standard error of the mean.