Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 25;13:525–536. doi: 10.2147/IJN.S149532

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Jeong et al. This work is published and licensed by Dove Medical Press Limited

The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

PMC Copyright notice

Antitumor activity of psi-hBCL2/polymers in HCT116 cells. (A) After complexing psi-hBCL2 (0.05, 0.1, 0.5, or 1 μg) and WSC or AEMA-g-WSC at the indicated w/w ratios, polyplexes were incubated with HCT116 cells for 48 h, after which cell viabilities were measured in CCK assays. (B) After a 48-h incubation of psi-hBCL2 (4 μg)/WSC or AEMA-g-WSC polyplexes at the indicated w/w ratios, caspase-3 expression was measured by immunocytometry. (C) After a 48-h incubation with the polyplexes, apoptosis was detected by annexin V-PE and PI staining by flow cytometry in HCT116 cells (a) control, (b) TurboFect, (c) WSC (1:32, w/w), (d) AEMA (1:16, w/w), (e) AEMA-g-WSC (1:4, w/w), (f) AEMA-g-WSC (1:8, w/w).

Abbreviations: AEMA-g-WSC, 2-aminoethyl methacrylate-grafted water-soluble chitosan; CCK, cell counting kit-8; PE, phycoerythrin; PI, propidium iodide.