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Primer sequences
| Gene | Forward primer sequence (5′–3′) | Reverse primer sequence (5′–3′) |
|---|---|---|
| Alp | GGCACAAGCACTCCCACTTT | TGGTCAATCCTGCCTCCTTC |
| Col-1A1 | AAGAACGGAGATGACGGAGAAG | GCACCATCCAAACCACTGAA |
| Opn | CACCGCAGAATGCTATGTCC | GTGGTCATCGTCCTCATCCTC |
| Runx-2 | GCAGGAGGAAAATGAGCAAAG | GTGAGTGAGCAGAGCCGAGA |
| Gapdh | CCACTTTGTGAAGCTCATTTCCT | TCGTCCTCCTCTGGTGCTCT |
