Figure 5.

Distribution of C9orf72 expansion-associated inclusions in Case 2’s preclinically resected lateral temporal lobe. Tissues sections were traced and subjected to exhaustive mapping at 60× magnification. (A) TDP-43 immunohistochemistry revealed numerous neurons lacking nuclear staining despite the absence of an apparent TDP-43 inclusion (arrowheads in photomicrographs and black dots in map). These neurons were most abundant in deep cortical layers. Only one neuron with a TDP-43 cytoplasmic inclusion was observed (red dot and map inset). (B–D) GA, GP and GR dipeptide repeat protein NCI were abundant. Numbers 2 and 6 denote cortical layers within section tracings. Scale bar = 25 µm.