Figure 7. Purified tumor cells and tumor vasculature express common endothelial markers.

(A) Venn diagram of genes upregulated in Tom⁺PECAM1⁻ and Tom⁺PECAM1⁺ cells compared to Tom⁻PECAM1⁻ cells isolated from AST tumors.

(B) Gene ontology analysis of 32 overlapping genes from (A).

(C) Gene expression of endothelial genes identified in (A) by real-time PCR. Data shown are normalized to Actb expression and expressed relative to sorted Tom⁻PECAM1⁻ cells (n = 3, mean ± SEM).

(D, E) Representative H&E staining (D) and immunostaining (E) for Tomato (red), KDR (green) and DAPI (blue) on sagittal section of E9.5 AT embryo (n = 2). Scale bars, 250 μm.

(F) Magnification of boxed inset in (E). Scale bars, 100 μm.

(G) Magnification of boxed inset in (F) with high magnification inset in lower left. Scale bars, 50 μm.

(H, I) Representative H&E staining (H) and immunostaining (I) for Tomato (red), KDR (green) and DAPI (blue) on sagittal section of E10.5 AT embryo (n = 4). Scale bars, 250 μm.

(J) Magnification of boxed inset in (I). Scale bars, 100 μm.

(K) Magnification of boxed inset in (J) with high magnification inset in lower left. Scale bars, 50 μm.

(L) Representative immunostaining of Tomato positive proliferations in sagittal sections of E15.5 AST embryo (Figure 6H–I). Tomato (red), KDR (green), DAPI (blue)(n = 4). High magnification insets are shown in upper right. Scale bar, 10 μm.

*p < 0.05, ** p < 0.01, *** p < 0.001.

See also Table S3.