Fig. 2.
Accumulation of IP1 by increasing concentrations (10−10 to 10−7 M) of human and mouse kisspeptin-10 (hkiss-10 and mkiss-10, respectively) as measured by the IP-One HTRF™ assay in the CHO-GPR54 cell line as percent inhibition of the fluorescence resonance energy transfer (FRET) signal. Compounds and reagents were added by the Biomek FX robotic workstation in high-throughput screening format.