Table 2. Tissue vRNA burden, ISH and mFISH results.
| Tissue Source | Tissue Name | vRNA copies/mg | ISH | mFISH |
|---|---|---|---|---|
| Maternal | Mesenteric LN | 9763.8 | - | |
| Spleen | 981.9 | + | + | |
| Uterus | 1471.7 | + | + | |
| Decidua | 278.2 | + | + | |
| Fetal | Amniotic/Chorionic Membrane | 4933.7 | + | + |
| Colon | 355157.0 | + | + | |
| Stomach | 6140.4 | - | ||
| Pericardium | 1196.3 | - | ||
| Adipose Tissue-Omentum | 5102.2 | + | - | |
| Lung | 37947.0 | + | + | |
| Seminal Vesicle/Prostate | 5700.0 | - | ||
| Testis | 5188.1 | - | ||
| Eye (Retina/Cornea/Sclera) | 316.5/135.1/370.8 | - |
Tissues with a detectable vRNA burden were evaluated by ISH and mFISH. ISH detects positive sense vRNA; mFISH detects ZIKV replicative intermediates (negative and positive sense vRNA).
“+” indicates detectable signal in these tissues sections,
“-”indicates no signal. Tissues with no detectable ISH signal were not further evaluated by mFISH.