Table 2. Primers used in the present study for DNA barcoding analyses.
Locus | Primer name | Sequence (5’-3’) | Ta | Primer note | Reference |
---|---|---|---|---|---|
rbcL | rbcLa_F | ATG TCA CCA CAA ACA GAG ACT AAA GC | 55 | Universal | [35] |
rbcLajf634R | GAA ACG GTC TCT CCA ACG CAT | Universal | [36] | ||
CS_rbcL-300rev | TAA AGG ATA CGC TAC ATA AGC | 55 | Specific for Camellia sinensis; used to check the SNP-M (68 bp) in sequencing PCR | In the present study | |
matK | 1R_KIM-f | ACC CAG TCC ATC TGG AAA TCT TGG TTC | 55 | Universal | Ki-Joong Kim, pers. comm. |
3F_KIM-r | CGT ACA GTA CTT TTG TGT TTA CGA G | ||||
trnH(GUG)-psbA | psbA3'f | GTT ATG CAT GAA CGT AAT GCT C | 55 | Universal | [37] |
trnHf* | CGC GCA TGG TGG ATT CAC AAT CC | [38] | |||
Can_3psb-for | GTT TCT TCT GTA CCA ACC TGC | 56 | Specific for Cinnamomum verum and C. cassia (cinnamon) | In this study | |
Can_3trnH-rev | TAC TTC ACT CTC CTT CCT GCT | ||||
Citrus_psbA-in | GTA TTG ATC CGT TAT TTA GTC G | 57 | Specific for Citrus sp. (lemon, orange, grapefruit, and lime) | In this study | |
Citrus_trnH-in | ATC TTA TCT TAC TTA TGA AGA ACC | ||||
Glycyrrhiza_psbA-in | GTT TTA AAG AAG GAT ACG AGG | 55 | Specific for Glycyrrhiza glabra (licorice) | In this study | |
Glycyrrhiza_trnH-inA | TAC ATT CGC CCT TCT TAT AC | ||||
Mentha_2psb_for | TTC CAG GCA AGT CCA ATA CT | 56 | Specific for Mentha sp. (M. aquatica, M. arvensis, M. canadensis, M. longifolia, P. pulegium, M. suaveolens, M. pulegium, and M. x piperita, M. spicata) | In this study | |
Mentha_2trnH_rev | TGT GTA GGA GTT TTT GAA AAT AGA C | ||||
Piminella_psbA-in | ACC TAG CTG CTG TTG AAG CTC | 60 | Specific for Pimpinella anisum (anise) | In this study | |
Pimpinella_trnH-in | GGA GCA ATA TCG CTT TCT TGA TAG A | ||||
rps7-trnV(GAC) | CS_rps7_1 | GTG CTA TGG CTC GAA TCC GT | 67 | Specific for Camellia sp.; used to discriminate C. sinensis from C. pubicosta | In this study |
CS_trnV(GAC)_1* | ACC ACG TCA AGG TGA CAC TC | ||||
P6 loop trnL(UAA) | g | GGG CAA TCC TGA GCC AA | 55 | Universal | [39] |
h* | CCA TTG AGT CTC TGC ACC TAT C | ||||
ITS2 | S2F | ATG CGA TAC TTG GTG TGA AT | 55 | Universal | [40] |
S3R* | GAC GCT TCT CCA GAC TAC AAT | ||||
BELL-1 | GGD GCG GAK AHT GGC CYC CCG TGC | 55 | Universal | [41] | |
BELL-3 | GAC GCT TCT CCA GAC TAC AAT |
*, labeled