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. 2018 Jan 30;13(1):e0192334. doi: 10.1371/journal.pone.0192334

Table 2. Primers used in the present study for DNA barcoding analyses.

Locus Primer name Sequence (5’-3’) Ta Primer note Reference
rbcL rbcLa_F ATG TCA CCA CAA ACA GAG ACT AAA GC 55 Universal [35]
rbcLajf634R GAA ACG GTC TCT CCA ACG CAT Universal [36]
CS_rbcL-300rev TAA AGG ATA CGC TAC ATA AGC 55 Specific for Camellia sinensis; used to check the SNP-M (68 bp) in sequencing PCR In the present study
matK 1R_KIM-f ACC CAG TCC ATC TGG AAA TCT TGG TTC 55 Universal Ki-Joong Kim, pers. comm.
3F_KIM-r CGT ACA GTA CTT TTG TGT TTA CGA G
trnH(GUG)-psbA psbA3'f GTT ATG CAT GAA CGT AAT GCT C 55 Universal [37]
trnHf* CGC GCA TGG TGG ATT CAC AAT CC [38]
Can_3psb-for GTT TCT TCT GTA CCA ACC TGC 56 Specific for Cinnamomum verum and C. cassia (cinnamon) In this study
Can_3trnH-rev TAC TTC ACT CTC CTT CCT GCT
Citrus_psbA-in GTA TTG ATC CGT TAT TTA GTC G 57 Specific for Citrus sp. (lemon, orange, grapefruit, and lime) In this study
Citrus_trnH-in ATC TTA TCT TAC TTA TGA AGA ACC
Glycyrrhiza_psbA-in GTT TTA AAG AAG GAT ACG AGG 55 Specific for Glycyrrhiza glabra (licorice) In this study
Glycyrrhiza_trnH-inA TAC ATT CGC CCT TCT TAT AC
Mentha_2psb_for TTC CAG GCA AGT CCA ATA CT 56 Specific for Mentha sp. (M. aquatica, M. arvensis, M. canadensis, M. longifolia, P. pulegium, M. suaveolens, M. pulegium, and M. x piperita, M. spicata) In this study
Mentha_2trnH_rev TGT GTA GGA GTT TTT GAA AAT AGA C
Piminella_psbA-in ACC TAG CTG CTG TTG AAG CTC 60 Specific for Pimpinella anisum (anise) In this study
Pimpinella_trnH-in GGA GCA ATA TCG CTT TCT TGA TAG A
rps7-trnV(GAC) CS_rps7_1 GTG CTA TGG CTC GAA TCC GT 67 Specific for Camellia sp.; used to discriminate C. sinensis from C. pubicosta In this study
CS_trnV(GAC)_1* ACC ACG TCA AGG TGA CAC TC
P6 loop trnL(UAA) g GGG CAA TCC TGA GCC AA 55 Universal [39]
h* CCA TTG AGT CTC TGC ACC TAT C
ITS2 S2F ATG CGA TAC TTG GTG TGA AT 55 Universal [40]
S3R* GAC GCT TCT CCA GAC TAC AAT
BELL-1 GGD GCG GAK AHT GGC CYC CCG TGC 55 Universal [41]
BELL-3 GAC GCT TCT CCA GAC TAC AAT

*, labeled