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. 2017 Dec 14;7(3):e1408748. doi: 10.1080/2162402X.2017.1408748

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Figure 3. — Immunofluorescence of MLH1^−/⁻ tumors and IFNγ–ELISpot after prophylactic vaccination. (A) Tumor microenvironment was studied from GIT cryostat sections of 4µm. Analyses were done on a laser scanning microscope (Zeiss) using 20x objectives. (B) Reactivity of PBL (during vaccination) or splenocytes (endpoint) against target cells (MLH1^−/− 7450 T1 M1, MLH1^−/− 328, and YAC-1) was examined after overnight co-incubation. Lymphocytes were isolated from vaccinated and control mice at different time points. Experiments revealed increased reactivity upon vaccination. Values are given as mean ± SD.