Figure 3.

The pro-inflammatory milieu induced by alloDCs recruits innate immune cells to the site of injection. (A) C57BL/6NRj mice were injected i.d. with 1×10⁶ alloDCs, along with 20 μg CFSE (green fluorescent cell-membrane dye) to be able to identify the injection site. Recruitment of innate immune cells at the site of injection was analyzed after 48 hours. (B-E) Recruitment of NK1.1⁺ NK cells (B, C) and Gr1⁺ neutrophils (D, E) to the site of injection in response to injected alloDCs was analyzed by immunofluorescent staining. Representative staining of one mouse per group is shown in B and D (green = injected area, blue = nuclei, red = NK1.1 or Gr1). Quantification of infiltrated NK-cells and neurophils (C, E) was determined as number of positive cells per mm². Data are shown as mean±SEM (* P<0.05; ** P<0.01; *** P<0.001).