Figure 1.

Spi6 expression is upregulated after allogeneic T cell activation. (A) MLR was performed using C57 BL/6 WT or Spi6−/− pan T cells as responders and BALB/c BMDCs as stimulators with a ratio of 5:1. After co-culture for 3 days, cells were stained with anti-mouse GzmB and Spi6 antibodies and analyzed by flow cytometry. Representative dot plots are gated on total live T cells (Live/Dead⁻ TCRβ⁺CD4⁺ or CD8⁺). (B-E) BALB/c mice were lethally irradiated on day -1. On day 0, hosts were injected with 3 × 10⁶ TCD-BM cells alone or combined with 1 × 10⁵ WT or Spi6^−/− pan T cells isolated from C57 BL/6 donor mice. On day 7, total splenocytes were harvested from host mice and analyzed by flow cytometry. Representative dot plots (B) are gated on total live donor T cells (Live/Dead⁻ H-2Kb⁺ TCRβ⁺CD4⁺ or CD8⁺) and summary data of GzmB (C), Spi6 (D) expression and co-expression (E) from 3 independent experiments are shown as the percentages of total donor CD4⁺ or CD8⁺ cells. Unpaired student T tests were performed to determine statistical significance.