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. 2018 Jan 30;7:e32237. doi: 10.7554/eLife.32237

Figure 4. Astrocytes release glutamate and ATP/adenosine in response to neuronal activity.

(A) Synaptic parameters vs time in the presence of CPT (2 µM) or LY367385 (100 µM). Zero time correspond with the beginning of the IN depolarization train (arrow). The open circles show the synaptic parameters measured during the IN depolarization train. (B) Relative changes in synaptic parameters during and after the IN depolarization train in the presence of CPT (2 µM; n = 7) or LY367385 (100 µM; n = 4). (C) Calcium event probability vs time in the presence of CPT (2 µM) or LY367385 (100 µM). The pink area corresponds with the duration of the stimulus. (D) Changes in the calcium event probability 15 s before (basal) and 15 s after the stimulation started in the presence of CPT (2 µM; n = 109 astrocytes) or LY367385 (100 µM; n = 96 astrocytes). Data are represented as mean ± s.e.m., *p<0.05, **p<0.01, ***p<0.001.

Figure 4.

Figure 4—figure supplement 1. Astrocytes co-release glutamate and ATP.

Figure 4—figure supplement 1.

(A) Number of cells that underwent synaptic potentiation, depression, both or no change after IN depolarization. (B) Synaptic parameters vs time in control conditions (n = 5) and in the presence of CPT (2 µM; n = 7) or LY367385 (100 µM; n = 4). Zero time corresponds with the beginning of the IN depolarization train. Open circles show the synaptic parameters measured during the IN depolarization train. (C) Potentiation duration and time to maximum potentiation in control conditions and in the presence of CPT (2 µM). (D) Depression duration and time to maximum depression in control conditions and in the presence of LY367385 (100 µM). Data are represented as mean ± s.e.m., *p<0.05, **p<0.01.