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. 2017 Dec 26;7(3):e1407897. doi: 10.1080/2162402X.2017.1407897

Figure 2.

Figure 2.

IFN-γ response of the CD4+ T cell clone NY67. (A) Nucleotide and amino acid sequences of the CDR3 of the beta and alpha chains of the TCR of the T cell clone NY67. (B) Melanoma cell lines were cultured in the presence or absence of IFN-γ and then loaded or not with the NY-ESO-1(157-170) peptide and washed. They were then cultured for 5 h with the CD4+ T cell clone NY67. IFN-γ response of the clone was measured by flow cytometry after CD4/IFN-γ staining. (C) Results are expressed as the mean ± SEM of the percentage of IFN-γ producing CD4+ T cells obtained from three independent experiments.