Figure 3.

Melanoma cell lines are sensitive to the oncolytic activity of MV. (A) M6 and M18 melanoma lines were cultured alone (NI, not infected) or infected by MV-eGFP at an MOI of 2 (MV). Pictures were taken every 6 h during the next 72 h. (B) M6 and M18 cell lines were cultured alone (NI) or infected by MV-eGFP at MOI of 2 (MV). After 72 h, the percentage of GFP+ cells was measured by flow cytometry. Indicated value are means ± SEM of three independent experiments. (C) M6 and M18 cell lines were cultured alone (NI) or infected by MV at MOI of 2 (MV). After 72 h cells were labeled with Annexin V-APC and propidium iodide (PI). Percentage of Annexin V+ and/or PI+ cells was measured by flow cytometry. Indicated values are means ± SEM of three independent experiments.