Figure 7.

Sensitivity of NY-ESO-1 donor melanoma cell lines M6 and M18 to six different OV. M6 and M18 melanoma cell lines were infected with VV (MOI = 0.1), VSV (MOI = 0.01), HSV (MOI = 1), AdV (MOI = 100) during 72 h. (A) Pictures were taken every 6 h. (B) 72 h later, the percentage of GFP+ cells for MV-eGFP, VV, VSV and HSV, or RFP+ cells for AdV was measured by flow cytometry. (C and D) M6 and M18 melanoma cell lines were infected with EV (MOI = 5). 72 h later, cells were stained with an antibody against VP-1 of EV and an Alexa-488 secondary antibody. Fluorescence was measured by flow cytometry. (C) Histogram plots of control (grey) and VP1 (white) stainings. (D) Results are expressed as mean ± SEM of three independent VP1 stainings. (E) M6 and M18 cell lines were infected with MV-eGFP (MOI = 2), VV (MOI = 0.1), VSV (MOI = 0.01), HSV (MOI = 1), AdV (MOI = 100) and EV (MOI = 5). 72 h later, cells were labeled with TO-PRO®-3. TO-PRO®-3+ cells were measured by flow cytometry. Indicated value are means ± SEM of three independent experiments.