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. 2018 Jan 9;7(3):e1395997. doi: 10.1080/2162402X.2017.1395997

Figure 1.

Figure 1.

Effects of CXCL11 transduction into Human CAR T cells. A) CXCL11 secretion: Human CAR T cells were transduced with the CXCL11 gene and placed into culture for 24 hours. As measured by ELISA assay, the CXCL11 transduced T cells secreted significantly (p < 0.001) more CXCL11. B) Chemotaxis: Activated human T cells were placed in the top of a Boyden chamber. In the lower well was placed cell media (R10) as a negative control, 10 ng/ml of recombinant human CXCL11 protein as a positive control, or conditioned media from the CAR/CXCL11 cells. Both recombinant CXCL11 and the CXCL11-conditioned media significantly (p<0.001) enhanced migration of human T cells compared to media control. C) CAR T cell Killing: Equal numbers of CAR or CAR/CXCL11 T cells were added to mesothelioma cells not expressing mesothelin (EMParental) or to mesothelioma cells expressing human mesothelin (EMMeso) at various T cell to tumor ratios. After overnight incubation, their ability to kill the target cells was determined.