Figure 1.

Germination and post-germination development of the elo3-6 mutant. (A) The Col-0 and elo3-6 seeds were sown on half-strength MS with 1% (w/v) sucrose, stratified for 48 h at 4°C, exposed to white light for 6 h to stimulate germination, shifted to darkness, and followed in time lapse with pictures taken every 1 h. The indicated time points refer to time after white light exposure. (B) Germination and post-germination stage percentage of Col-0 and elo3-6 seeds in darkness. The seeds were sown on half-strength MS without sucrose, stratified for 48 h at 4°C, exposed to white light for 6 h, and shifted to darkness. Fifty seeds were taken at the indicated time points. No germination, germination, and post-germination stages were observed under the stereomicroscope and quantified. (C) Col-0 and elo3-6 seedlings were grown 4 days on half-strength MS medium with (+) or without (−) sucrose in darkness. Bars represent mean values ± s.d. Differences between mutant and wild type were statistically analyzed with an unpaired two-tailed Student's t-test and significant differences are indicated with asterisks (P<0.05).