FIG. 3.

(A) Five-gene cluster in Mtb H37Rv encoding EGT biosynthetic enzymes; (B) Three protein domains common in egt1 homologs of fungi. (A) An example of the EGT five-gene cluster responsible for EGT biosynthesis that has only been found in Actinobacteria (51, 52). (B) N. crassa Egt1 is a multidomain protein, with an N-terminal SAM-dependent methyltransferase, with 27% identity to M. smegmatis EgtD and C-terminal DinB_2 and FGE-sulfatase domains that have 24% identity to M. smegmatis EgtB (modified from Ref. 9). The domains of NcEgt1 corresponding to Mtb EgtB and EgtD have been indicated. SAM, S-adenosylmethionine.