Table 2.

Sequence of Control Antisense Oligonucleotides

No.	Group	Chemistry	Sequence (5′-3′)	Tm (°C)
CM-PO	Controls	PO, LNA	GdCdTAdTdACdCdAGdCdGTdCdGTdCdATd	—
CM-PS		PS, LNA	GdCdTAdTdACdCdAGdCdGTdCdGTdCdATd	—
MM-1		MOE, 2′-FRNA, OMe, PS	P-TsTstCstTscTsaAsaTscTstsCstsTscsAsA	56.1
MM-2		MOE, 2′-FRNA, OMe, PS	P-TsCstTscTstCsaAsaTstCstsTscsTstsAsA	57.8
MM-3		MOE, 2′-FRNA, OMe, PS	P-TsTscTstCstTsaAsaCstTscsTstsCstsAsA	56.3

All oligonucleotides used PS modification unless mentioned. MM1–3 have PO backbones except the labeled PS backbones.

Bold: LNA modification, bold italic: 2′-O-MOE, underline: 2′-F RNA, lowercase: 2′-O-Me, s: phosphorothioate, P: phosphate, d: deoxyribose.

LNA, locked nucleic acid; PS, phosphorothioate.