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. 2017 Dec 14;9(3):3121–3130. doi: 10.18632/oncotarget.23213

Figure 6. Smad activity is irrelevant to NF-κB activity in TGFβ1-treated A7r5 cells.

Figure 6

The cells were transfected with 3TP-PAI1-Luc (A, B) or 3×kB-Luc (C, D) reporter constructs. Under the condition, the cells were co-transfected with Smad3-DN (A and C) or siSmad3 (B and D). The transfected cells were further treated with TGFβ1 (1 ng/ml) for 24 h. The luciferase activity was expressed as a relative value compared to that of the untreated cells which was set to 100%. The data were expressed as the mean ± SEM (n = 4). ***p < 0.005. n.s., not significant. (E, F) The cells were transfected with Smad3-DN or siSMAD3 for 48 h and then treated with TGFβ1 (1 ng/ml) for 1 h prior to western blot analysis. #GFP-Smad3.

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