Figure 2. Tubacin inhibits accumulation of FGFR3^K644E, MYC and cyclin D1 and selectively induces a DNA damage response in MEFs expressing FGFR3^K644E and/or MYC.

(A) Representative images of soft agar colonies formed by MEFs expressing FGFR3^K644E plus MYC that were treated with either vehicle (DMSO) or 20 µM tubacin, and HDAC6 KO MEFs expressing FGFR3^K644E plus MYC that were treated with DMSO. (B) The mean number of soft agar colonies per plate is shown (n = 3). ^**p < 0.0005. (C) MEFs transfected with empty expression vector (vector) or expressing FGFR3^K644E, MYC, or both FGFR3^K644E and MYC as indicated were treated with DMSO (D) or 20 µM tubacin (T) for 16 hours before cells were collected and lysed. Immunoblots were performed for the indicated proteins. ^* ectopic MYC, - endogenous MYC. For pRBSer807/811, the asterisk indicates the primary band specific for pRbSer807/811. GAPDH was used as a loading control.