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. 2017 Dec 14;9(3):3188–3197. doi: 10.18632/oncotarget.23225

Figure 4. siSmad4 inhibits HIF-1α in hypoxia.

Figure 4

(A) qRT-PCR showing clear knockdown of Smad4 by siSmad4 transfection for 48 h. (B) 24 h after transfection, HFFs and HKFs were treated with 1% hypoxia or normoxia for 24 h. 48 h after transfection, the expression of mRNA level of HIF-1α was down-regulated in the group silencing Smad4. (C) After 72 h transfected by si-Smad4 and 24 h hypoxia treatment, the protein level of HIF-1α was down-regulated in the si-Smad4 group compared with the negative control group. The histogram shows the protein band intensity ratio of HIF-1α to β-Tubulin.