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. 2017 Dec 15;9(3):3417–3431. doi: 10.18632/oncotarget.23283

Figure 6. ZNF224 mediates AG490-dependent transcriptional repression on c-Myc.

Figure 6

(A) K562 cells were treated with AG490 for 24h or vehicle only (DMSO) as control (−). Cell death was evaluated by annexin V staining followed by flow cytometry. Results represent the means +/− SD of two independent experiments. (B) K562 cells were treated with AG490 for 10 hours or vehicle only (DMSO) as control (−). c-Myc mRNA levels were measured by RT-qPCR. Error bars represent standard deviations of two independent experiments. (C) ZNF224 and c-Myc protein levels were measured by western blot analysis. G3PDH was used as loading control. One representative blot out of two performed is shown. (D) K562 cells were transiently transfected with DEL-6 or DEL-6-MUT constructs and treated with AG490 or vehicle only (DMSO) as control (−); after 10 h, luciferase activity was determined by normalizing Firefly to Renilla luciferase activity. Error bars represent standard deviations of two independent experiments. (E) shE7 and shGFP cells were treated with AG490 or vehicle only (DMSO) as control for 10 hours. c-Myc mRNA levels were measured by RT-qPCR. Relative amounts of c-Myc mRNA levels in shE7 and shGFP cells treated with AG490 were compared to those in shE7 and shGFP treated with DMSO (control). Error bars represent standard deviations of two independent experiments. (F) ZNF224 and c-Myc protein levels were measured by western blot analysis. Arrow indicates specific band. β-tubulin was used as loading control. One representative blot out of two is shown.