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. 2017 Dec 15;9(3):3417–3431. doi: 10.18632/oncotarget.23283

Figure 7. ZNF224 is involved in AG490-induced cell death of K562 Ima-R cells.

Figure 7

K562 Ima-R cells were exposed to AG490 or vehicle only (DMSO) as control (−) for 48 hours. (A) Cell death was determined by annexin V staining followed by flow cytometry. Error bars represent standard deviations of three independent experiments. (B) Caspase activity was biochemically measured. (C) ZNF224 and c-Myc mRNA levels were measured by RT-qPCR. Error bars represent standard deviations of three independent experiments. **p < 0.005 (left panel). ZNF224 and c-Myc protein levels were measured by western blot analysis. β-actin was used as loading control. One representative blot out of two is presented (right panel). (D) K562 Ima-R cells were silenced with two different siRNAs versus ZNF224 (siRNA ZNF224#1 or siRNA ZNF224#2) or with a control siRNA (siLuc) and after 48 hours were collected or exposed to 30 µM AG490 or vehicle only (DMSO) as control (−) for 30 hours. ZNF224 and c-Myc protein levels were evaluated by western blot analysis. β-actin was used as loading control. One representative blot out of two is presented (left panel). Cell death was determined by annexin V staining followed by flow cytometry. Error bars represent standard deviations of three independent experiments. **p < 0.005, (right panel).