(A–E) Demonstrating the immunohistochemical (IHC) microscopic finding (400x) for identification of cellular expression of CD3 (brown color). (F). Analytical result of number of CD3+ cells, * vs. other groups with different symbols (†, ‡, §), p < 0.0001. (G–K) Demonstrating IHC microscopic finding (400×) for identification of cellular expression of CD4 (brown color). (L) Analytical result of number of CD4+ cells, * vs. other groups with different symbols (†, ‡, §), p < 0.0001. The scale bars in right lower corner represent 20 µm. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test (n = 6). Symbols (*, †, ‡, §) indicate significance (at 0.05 level). NC = normal control; SC = sham control; BDEX = brain death extract; Mel = melatonin; Luz = luzindole.