Figure 6. TN mice were re-challenged with 1 × 10⁵ cells in the left flank.

(A) As for the first immunization protocol, animals were monitored every 2 days for tumor growth and sacrifice occurred when tumor volume reached 1500 mm³. When the tumor reached the cutoff in the first animal (day 26), tumor volume in all the different groups was compared. (B) Kaplan-Meyer curve showing the percentage of survival of animals in the experimental groups. Days are from the re-challenge. Evaluation of CD4⁺ CD25⁺FoxP3 T regulatory cells (C) and T_CD4⁺/T_reg and T_CD8⁺/T_reg ratio (D and E) in the blood of each re-challenged animal at day 0 (green) and at sacrifice (blue).