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. 2017 Dec 17;9(3):3794–3804. doi: 10.18632/oncotarget.23376

Figure 2. BA inhibits breast cancer cells 4T1 and MDA-MB-231 migration and invasion.

Figure 2

(A) Tumor cells were seeded in six-well plates. We make a ’wound’ after the cells grew ∼90% confluence. After incubation for 48 h, the groups were graphed. The black lines indicate the section occupied by the initial scraping, and migrated cells were quantified. (B) Tumor cells were seeded in the roof chamber of transwell with serum-free medium and treated with vehicle or different concentrations of BA. After 48 h, migrated cells were fixed, stained and graphed (20×) and quantified. (C) BA inhibits 4T1 and MDA-MB-231 invasion. Tumor cells were treated with different concentrations of BA and invaded through Matrigel. Invaded cell number was counted (*P < 0.05; **P < 0.01; ***P < 0.001). (D, E) 4T1 and MDA-MB-231 cells were treated with different concentrations of BA. After 48 h, cells were harvested, and western blot assay was performed to detect the expression of MMP-2, MMP-9, TIMP-2, Stat3, P-Stat3, FAK, P-FAK. β-actin served as loading control.