Table 2. TILs culture in vitro and CD4/CD8 composition of expanded TILs in 8 SCCHN patients.

Patient No	No. of missense mutations	No. of HLA-A restricted neoantigen candidates (the affinity of <500 nM)	No. of cells at the starting point	No. of expanded TILs	Expanded TILs
					CD4 (%)	CD8 (%)
B1	51	7	N/A	N/A	N/A	N/A
B2	1779	251	1×105CD8+cells	8.0×107	N/A	93.0
B3	11	0	6×106 single cells	9.0×108	25.7	67.5
B4	48	4	1×105CD8+cells	8.3×107	N/A	95.7
B5	4290	532	6×106 single cells	2.4×108	84.0	11.4
B6	49	19	6×106 single cells	5.1×107	34.5	42.3
B7	78	14	6×106 single cells	2.0×109	39.5	56.5
B8	901	273	6×106 single cells	1.8×109	34.4	58.0

N/A: Not applicable.