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. 2017 Dec 12;9(3):3830–3841. doi: 10.18632/oncotarget.23237

Figure 4. Rab3D is a direct target of miR-27b.

Figure 4

(A) The predicted sites of miR-27b binding to the 3′-UTR region of Rab3D were detected using bioinformatics prediction tools. The mutated site in the 3′-UTR region of Rab3D was also shown (B–E). The mRNA and protein level of Rab3D were detected in the presence of miR-27b mimics (B–D) or miR-193b inhibitor (C, E) in CRC cells. (F) Spearman’s correlation analysis between miR-27b and Rab3D mRNA levels in 80 CRC patients (G–H). The luciferase activity after transfection with the luciferasereporter plasmid containing either wild type or mutant Rab3D 3′-UTR in the presence of miR-27b mimic or negative controls in SW480 and SW1116 cells. Results shown are the mean ± SEM (**P < 0.01, *** P < 0.001) of triplicate determination from three independent experiments.