Figure 2. Relative expression of transcripts encoding each antigen in the B-ALL patients compared to the healthy volunteers.

Dots indicate the ΔC_T values, whereby the C_T of the endogenous control GAPDH is subtracted from the C_T of each gene. Genes that were not expressed were assigned a C_T value of 40. The higher the ΔC_T value, the less antigen was expressed. All ΔC_T values for antigens were lower level than the reference gene GAPDH. Streaked dots, representing patient sample ALL004, were outliers that do not represent antigen expression. P-values were determined using a two-way ANOVA test. Ns, not significant.