Table 4. Immunoreactivity score for the antigens SSX2, survivin, SSX2IP and WT1 as detected by ICC in B-ALL patient samples and healthy donors.
| Patient number | Cells only | Controls# | Isotype Rabbit | Actin | SSX2 | SSX2IP | Survivin | WT1 |
|---|---|---|---|---|---|---|---|---|
| ALL001 | 0 | 0 | 0 | 96 | 83.5 (mod) | 89.5 (mod) | 82.5 (mod) | 94.5 (mod) |
| ALL002 | 0 | 0 | 0 | 300 | 196 (high) | 99.5 (mod) | 89.5 (mod) | 200 (high) |
| ALL003 | 0 | 0 | 0 | 98.5 | 187 (high) | 200 (high) | 98 (mod) | 92.5 (mod) |
| ALL004 | 0 | 0 | 0 | 94.5 | 91 (mod) | 96 (mod) | 95.5 (mod) | 84.5 (mod) |
| ALL005 | 0 | 0 | 0 | 198 | 188 (high) | 178 (high) | 93.5 (mod) | 95.5 (mod) |
| ALL006 | 0 | - | 0 | 200 | 200 (high) | 198 (high) | 95.5 (mod) | 96 (mod) |
| ALL007 | 0 | 0 | 0 | 200 | 198 (high) | 200 (high) | 186 (high) | 96 (mod) |
| ALL008 | 0 | 0 | 0 | 200 | 84 (mod) | 91 (mod) | 89.5 (mod) | 79 (mod) |
| ALL009 (BM) | 0 | 0 | 0 | 197 | 188 (high) | 176 (high) | 195 (high) | 181 (high) |
| ALL009 (PB) | 0 | 0 | 0 | 200 | 171 (high) | 90.5 (mod) | 175 (high) | 196 (high) |
| ALL010 (BM) | 0 | 0 | 0 | 99 | 93 (mod) | 95 (mod) | 89 (mod) | 99 (mod) |
| ALL010 (PB) | 0 | 0 | 0 | 97.5 | 181 (high) | 200 (high) | 100 (mod) | 100 (mod) |
| Total | 0/10 | 0/10 | 0/10 | 10/10 | 10/10 | 10/10 | 10/10 | 10/10 |
| HV003 | 0 | 0 | 0 | 273 | 0 | 0 | 0 | 35.5 (mod) |
| HV004 | 0 | 0 | 0 | 198 | 151.5 (high) | 38 (mod) | 0 | 0 |
| HV007 | 0 | 0 | 0 | 267 | 0 | 0 | 0 | 0 |
| HV008 | 0 | 0 | 0 | 187 | 0 | 0 | 0 | 0 |
| HV016 | 0 | 0 | 0 | 288 | 0 | 0 | 0 | 64.5 (mod) |
| HV021 | 0 | 0 | 0 | 189 | 0 | 0 | 0 | 0 |
| Total | 0/6 | 0/6 | 0/6 | 6/6 | 1/6 | 1/6 | 0/6 | 2/6 |
Actin acted as a positive control. #The two isotype antibodies were used as negative controls or one isotype control and one test with no primary antibody. Immunoreactivity scores are as follows:- 0 = negative; 1–29:weak; 30–143: moderate (mod) and 144–228: high; >228: very high. Data is representative of at least two independent experiments.