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. 2017 Dec 17;9(3):3908–3921. doi: 10.18632/oncotarget.23386

Figure 4. Immunoprecipitation of acetylated proteins and drug sensitivity of ERCC1-deficient cells.

Figure 4

(A) J45.01 cells were exposed to drugs for 48 hrs and acetylated proteins in the nuclear extracts were immunoprecipitated using anti-acetylated lysine antibody as described under Materials and Methods. Approximately 5% of the input for immunoprecipitation was used for Western blot analysis. (B) Chinese hamster ovary cell lines AA8 (wild type) and UV20 (ERCC1-deficient) were exposed to the indicated drugs for 48 hrs and analyzed for proliferation by MTT assay (upper panel). Untreated cells were analyzed by Western blotting to show differences in expression of DNA repair proteins (lower panel). Results are representatives of two independent experiments. IP, immunoprecipitate; Npb, niraparib; DAC, decitabine; Rom, romidepsin.