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. 2017 Dec 4;9(3):3922–3935. doi: 10.18632/oncotarget.22883

Figure 4. PPP1R14C RNAi depletion prevents cell cycle associated AR phosphorylation, reducing the proliferative and migratory capacity of prostate cancer cells.

Figure 4

(A) Western blot analysis of AR phosphorylation status at S81, S213 and S515 following AR immunoprecipitation in LNCaP cells depleted of PPP1R14C. (B) Nuclear-cytoplasmic localization as determined by western blot following nuclear-cytoplasmic fractionation of LNCaP cells depleted of PPP1R14C. (C) Western blot analysis of AR protein expression following PPP1R14C RNAi depletion in LNCaP cells cultured in full media and subsequently incubated with 20 µg/ml cycolheximide. (D) Impact of AKT inhibition (1 µM MK2206) on PSA mRNA expression in LNCaP cells cultured in full media and depleted of PPP1R14C. Data represents 3 independent experiments ± SEM. P- values were determined by unpaired student t test (**, * denote P-values of < 0.001 and < 0.01 respectively).