Table 1. Proteins identified in tumor tissue but not in normal tissue.
| Protein name | Accession number | MW (kDa) | Peptides identified | Protein coverage | Tissue origin* |
|---|---|---|---|---|---|
| Serpin B5 (Maspin) | SPB5_HUMAN | 42 | 18 | 47% | 1/2/2L |
| Leucine aminopeptidase 3 (LAP3) | AMPL_HUMAN | 53 | 11 | 27% | 1/2/2L |
| Annexin A3 (ANXA3) | ANXA3_HUMAN | 36 | 9 | 35% | 1/2/2L |
| Proteasome subunit alpha 1 (PSMA1) | PSA1_HUMAN | 30 | 9 | 34% | 1/2/2L |
| Septin-11 | D6RER5_HUMAN | 50 | 14 | 26% | 1/2/2L |
| Isoform 2 of Annexin A2 | ANXA2_HUMAN | 40 | 22 | 46% | 1 |
| Actin-related protein 2 | ARP2_HUMAN | 45 | 6 | 19% | 1 |
| Annexin A1 | ANXA1_HUMAN | 39 | 5 | 19% | 1 |
| Malate dehydrogenase, mitochondrial | MDHM_HUMAN | 36 | 5 | 19% | 1/2 |
| Gamma-glutamyl byfrolase | GGH_HUMAN | 36 | 5 | 15% | 1 |
| Glutaredoxin-3 | GLRX3_HUMAN | 37 | 4 | 17% | 1 |
| HLA class I histocompatibility antigen, A-43 alpha chain | IA43_HUMAN | 41 | 4 | 18% | 1 |
| Cathepsin B | CATB_HUMAN | 38 | 3 | 14% | 1 |
| EGF containing fibulin-like extracellular matrix protein | B4DW75_HUMAN | 39 | 3 | 10% | 1 |
| F-actin-capping protein subunit alpha-1 | CAZA1_HUMAN | 33 | 6 | 20% | 1/2L |
| Pyridoxal kinase | F2Z2Y4_HUMAN | 31 | 6 | 22% | 1 |
| AH receptor-interacting protein | A1P_HUMAN | 38 | 6 | 22% | 1 |
| Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | GBB1_HUMAN | 37 | 4 | 14% | 1 |
| Voltage-dependent anion-selective channel protein 1 | VDAC1_HUMAN | 31 | 3 | 13% | 1/2 |
| Isoform 2 of ficolin-3 | FCN3_HUMAN | 32 | 3 | 10% | 1 |
| Gamma-soluble NSF attachment protein | SNAG_HUMAN | 35 | 3 | 13% | 1 |
| Isoform 3 of filamin-binding LIM protein 1 | FBLI_HUMAN | 31 | 3 | 11% | 1 |
| Glutathione S-transferase P | GSTP1_HUMAN | 23 | 6 | 36% | 1 |
| Sp/P20160/CAP7_HUMAN Azurocidin | CAP7_HUMAN | 27 | 4 | 14% | 1 |
| Ras-related protein Rab-11A | RB11A_HUMAN | 24 | 3 | 15% | 1 |
| Peptidoglycan Recognition protein 1 | PGRP1_HUMAN | 22 | 5 | 32% | 1 |
| Complement factor D | CFAD_HUMAN | 27 | 4 | 20% | 1 |
| Alpha-enolase | ENOA_HUMAN | 47 | 36 | 42% | 1/2 |
| Protein disulfide isomerase family A, membrane 3, isoform CRA_bne | G5EA52_HUMAN | 55 | 27 | 38% | 1 |
| Isoform 2 of heat shock cognate 71kD protein | HSP7C_HUMAN | 54 | 14 | 28% | 1 |
| Procollagen c-endopeptidase enhancer 1 | PCOC1_HUMAN | 48 | 10 | 27% | 1 |
| Septin 6 | B1AMS2_HUMAN | 49 | 9 | 20% | 1 |
| Rab GDP dissociation inhibitor beta | E7EU23_HUMAN | 51 | 7 | 18% | 1 |
| Haptoglobin | HPT_HUMAN | 45 | 4 | 12% | 1 |
| Coronin | A7MAPO_HUMAN | 54 | 3 | 7% | 1 |
| Phosphoglycerate kinase 1 | PGK1_HUMAN | 45 | 3 | 11% | 1 |
| Isoform 3 of chitotriosidase-1 | CHITI_HUMAN | 49 | 3 | 8% | 1 |
| Isoform 3 of L-lactate dehydrogenase A chain | LDHA_HUMAN | 40 | 4 | 11% | 1 |
| Enlongation factor 1-alpha 1 | EF1A1_HUMAN | 50 | 4 | 9% | 1 |
| Acid ceramidase | E7EMM4_HUMAN | 42 | 4 | 11% | 1 |
| T-complex protein 1 subunit alpha | TCPA_HUMAN | 60 | 10 | 28% | 2 |
| Succinyl-CoA-Ketoacid coenzyme A transferase 1, mitochondrial | SCOT1_HUMAN | 56 | 13 | 38% | 2 |
| DnaJ homolog subfamily C member 3 | DnJC3_HUMAN | 58 | 13 | 35% | 2 |
| NOL1/NOP2/Sun domain family, member 2, isoform CRA_b | G3V1R4_HUMAN | 59 | 5 | 15% | 2 |
| UDP-N-acetylhexosamine pyrophosphorylase-like protein | UAP1L_HUMAN | 57 | 5 | 13% | 2 |
| Serine/threonine-protein kinase PAK2 | PAK2_HUMAN | 58 | 7 | 18% | 2 |
| Zyxin | B4DQR8_HUMAN | 52 | 3 | 11% | 2 |
| Golgi-associated PD2 and coiled-coil motif-contain protein | F5H1Y4_HUMA | 51 | 3 | 6% | 2 |
| Coronin-1B | COR1B_HUMAN | 54 | 3 | 6% | 2 |
| Annexin, AII | ANXII_HUMA | 54 | 4 | 29% | 2 |
| Thioredoxin reductase 1, cytoplasmic | B2R5P6_HUMAN | 55 | 7 | 17% | 2 |
| E3 ubiquitin-protein ligase XIAP | XIAP_HUMAN | 57 | 8 | 23% | 2 |
| Tripartite motif-containing protein 65 | TRI65_HUMAN | 57 | 5 | 21% | 2 |
| Nucleoprin p54 | B4DT35_HUMAN | 51 | 15 | 42% | 2 |
| D-3-phosphoglycerate dehydrogenase | SERA_HUMAN | 57 | 12 | 30% | 2 |
| Isoform 2 of Alpha-amino adipic semialdehyde dehydrogenase | AL7A1_HUMAN | 55 | 7 | 22% | 2 |
| Isoform 2 of Endophilin-B1 | SHLB1_HUMAN | 43 | 5 | 14% | 2 |
| Vasodilator-stimulated phosphoprotein | VASP_HUMAN | 40 | 5 | 17% | 2 |
| Endophilin-A2 | SH3G1_HUMAN | 41 | 3 | 10% | 2 |
| Uroporphyrinogen decarboxylase | DCUP_HUMAN | 41 | 3 | 10% | 2 |
| Short/branded chain specific acyl-CoA dehydrogenase, mitochondria | ACDSB_HUMAN | 47 | 3 | 9% | 2 |
| UPF0160 protein MYG1, mitochondrial | MYG1_HUMAN | 42 | 3 | 12% | 2 |
| Zinc finger cccH domain-containing protein | ZC3HF_HUMAN | 49 | 6 | 18% | 2 |
| Synaptosomal-associated protein 29 | SNP29_HUMAN | 29 | 4 | 22% | 2 |
| N-myc-interactor | NM1_HUMAN | 35 | 5 | 23% | 2 |
| HCG 2043421, isoform CRA-C | 134E3T4_HUMAN | 25 | 5 | 23% | 2 |
| Interferon-induced 35 kD protein | IN35_HUMAN | 32 | 3 | 21% | 2 |
| Chloride intracellular channel protein 3 | CLIC3_HUMAN | 27 | 3 | 13% | 2 |
| Putative WAS protein family homolog 4 (fragment) | H3BV49_HUMAN | 33 | 3 | 12% | 2 |
| DNA-directed RNA polymerase I, II, and III subunit | RPAB1_HUMAN | 25 | 7 | 41% | 2 |
| Putative ATP-dependent Clp protease protedytic subunit, mitchondrial | CLPP_HUMAN | 30 | 5 | 27% | 2 |
| Cathepsin G | CATG_HUMAN | 29 | 4 | 16% | 2 |
| Proteasome subunit beta type-4 | PSB4_HUMAN | 29 | 4 | 27% | 2 |
| 40S ribosomal protein S4 | RS4X_HUMAN | 30 | 4 | 19% | 2 |
| Autophage-related ptotein 101 | ATGA1_HUMAN | 25 | 4 | 19% | 2 |
| Tumor protein D52-like 2 | Q5JWU6_HUMAN | 25 | 3 | 17% | 2 |
| Microtubule-associated protein RP/EB family member | MARE1_HUMAN | 30 | 3 | 16% | 2 |
| Proteasome subunit alpha type | H0YL69_HUMAN | 26 | 3 | 17% | 2 |
| General transcription factor II | Q86U51_HUMAN | 30 | 3 | 14% | 2 |
| Obg-like ATPase 1 | J3KQ32_HUMAN | 47 | 10 | 25% | 2 |
| Phosphoglycerate kinase | B7Z7A9_HUMAN | 41 | 9 | 71% | 2 |
| 3-ketoacyl-CoA thidase, mitochondrial | YHIM_HUMAN | 42 | 12 | 29% | 2 |
| Flotillin-1 | FLOT1_HUMAN | 47 | 8 | 49% | 2 |
| Sepin H1 | SEEPH_HUMAN | 46 | 3 | 31% | 2 |
| Phosphoglycerate mutase 1 | PGAM1_HUMAN | 29 | 7 | 39% | 2 |
| Endoplasmic reticulum resident protein 29 | ERP29_HUMAN | 29 | 5 | 24% | 2 |
| U2 small nuclear ribonucleoprotein | RU2A_HUMAN | 28 | 3 | 39% | 2 |
| Ras association domain-containing protein | RASF3_HUMAN | 28 | 3 | 39% | 2 |
| Fructose-2, 6-bisphosphatase T1GAR | T1GAR_HUMAN | 30 | 6 | 27% | 2 |
| Galectin-3 | LEG3_HUMAN | 26 | 6 | 29% | 2 |
| Isoform 4 of Dehydrogenase/reductase SDR family member | DHRS4_HUMAN | 26 | 9 | 46% | 2 |
| Isoform 2 of V-type protein ATPase subunit E | VATE1_HUMAN | 24 | 3 | 18% | 2 |
| Mitotic checkpoint protein BUB3 | B4DDM6_HUMAN | 28 | 3 | 15% | 2 |
| DnaJhololog subunit C member | DNJC8_HUMAN | 30 | 3 | 14% | 2 |
| 14-3-3 protein gamma | 1433G_HUMAN | 28 | 3 | 13% | 2 |
| Peroxiredoxin-1 | PRDX1_HUMAN | 22 | 8 | 44% | 2 |
| Ras-related protein Rap-1A | RAP1A_HUMAN | 21 | 5 | 32% | 2 |
| High mobility group protein B2 | HMGB2_HUMAN | 24 | 3 | 25% | 2 |
| Isoform 4 o cellular nucleic acid-binding protein | CNBP_HUMAN | 20 | 3 | 21% | 2 |
| Stromal cell-derived factor 2 | SDF2_HUMAN | 23 | 3 | 22 | 2 |
| ADP-ribosylation factor-like protein 3 | ARL3_HUMAN | 20 | 3 | 27% | 2 |
| Transgelin | TAGL_HUMAN | 23 | 4 | 20% | 2 |
| Very long-chain-specific acyl-CoA dehydrogenase mitochondrial | F5H2A9_HUMAN | 73 | 14 | 25% | 2 |
| Prelamin-A/C | LMNA_HUMAN | 74 | 13 | 44% | 2 |
| Far upstreat element-binding protein 2 | FUB2_HUMAN | 73 | 7 | 14% | 2 |
| Far upstream element-binding protein 1 | FUBP1_HUMAN | 68 | 7 | 15% | 2 |
| Fibrinogen beta chain | F1BB_HUMAN | 56 | 3 | 51% | 2/2L |
| Isoform 2 of adenylyl cyclase-associated protein 1 | CAP1_HUMAN | 52 | 8 | 36% | 2 |
| Dihydrolopoyl dehydrogenase mitochondrial | DLDH_HUMAM | 54 | 8 | 25% | 2 |
| tRNA-splicing ligase RtcB homolog | RTCB_HUMAN | 55 | 7 | 35% | 2 |
| Elongation factor 1-alpha 1 | EF1A1_HUMAN | 50 | 3 | 26% | 2 |
| FAD_AMP lyase | H0YCY6_HUMAN | 55 | 3 | 8% | 2 |
| Isoform 2 of Ig mu chain C region | IGHM_HUMAN | 52 | 3 | 16% | 2 |
| Fascin | FSCN1_HUMAN | 55 | 7 | 22% | 2/2L |
| Isocitrate dehydrogenase | B4DFL2_HUMAN | 45 | 7 | 30% | 2 |
| Isoform 2 of Testin | TES_HUMAN | 47 | 4 | 27% | 2 |
| Isoform cytoplasmic of glutathione reductase | GSHR_HUMAN | 52 | 4 | 37% | 2 |
| Serpin H1 | SERPH_HUMAN | 46 | 3 | 24% | 2 |
| Septin-7 | E7EPK1_HUMAN | 51 | 3 | 8% | 2 |
| Fumarylacetoacetase | FAAA_HUMAN | 46 | 13 | 42% | 2 |
| Aspartate aminotransferase, cytoplasmic | AATC_HUMAN | 46 | 12 | 36% | 2 |
| Glutamine synthetase | GLNA_HUMAN | 42 | 6 | 12% | 2 |
| 26s protease regulatory subunit 10B | PRS10_HUMAN | 44 | 5 | 37% | 2 |
| Isovalery 1 Coenzyme A dehydrogenase | J3KR54_HUMAN | 47 | 4 | 26% | 2 |
| Medium-chain-specific acyl-CoA dehydrogenase, mitochondrial | B7Z9I1_HUMAN | 42 | 4 | 42% | 2 |
| Isoform 2 of alpha-1-antitrypsin | A1AT_HUMAN | 40 | 4 | 14% | 2 |
| 26s protease regulatory subunit 8 | A8K323_HUMAN | 45 | 4 | 15% | 2 |
| Isoform 2 of Selenium-binding protein 1 | SBP1_HUMAN | 45 | 4 | 12% | 2 |
| Isoform 2 of Putative N-acetylglucosamine-6-phosphate deacetylase | NAGA_HUMAN | 47 | 4 | 16% | 2 |
| Tubulin beta chain | E7EWR1_HUMAN | 40 | 6 | 33% | 2 |
| Alpha-centractin | ACT2_HUMAN | 43 | 3 | 19% | 2 |
| Isoform 2 of Transforming growth factor beta-1-induced transcript 1 protein | TGFI1_HUMAN | 48 | 3 | 13% | 2 |
| Plasmingen activator inhibitor | PAI1_HUMAN | 45 | 3 | 11% | 2 |
| Ig gamma-1 chain c region | 1GHG1_HUMAN | 36 | 14 | 53% | 2 |
| Poly(rC)-binding protein 1 | PCBP1_HUMAN | 37 | 6 | 24% | 2 |
| Isoform 2 of Arginase-1 | ARGI1_HUMAN | 36 | 6 | 25% | 2 |
| ANNEXIN | B4dt77_human | 38 | 5 | 20% | 2 |
| Fructose-bisphosphate aldolase c | ALDOC_HUMAN | 39 | 5 | 29% | 2 |
| Methionine adenosyltransferase 2 subunit beta | MATZB_HUMAN | 38 | 5 | 21% | 2 |
| Isoform 2 of 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial | HIBCH_HUMAN | 38 | 5 | 16% | 2 |
| Cysteine and histidine-rich domain-containing protein | CHRD1_HUMAN | 37 | 4 | 16% | 2 |
| Mitochondrial import receptor subunit TOM40 homolog | TOM40_HUMAN | 38 | 3 | 18% | 2 |
| Proliferation-associated protein 2G4 | F8VRZ3_HUMAN | 33 | 3 | 12% | 2 |
| Fructose-bisphosphate aldolase B | ALDOB_HUMAN | 39 | 3 | 13% | 2 |
| Poly-binding-splicing factor PUF60 | HOYEM1_HUMAN | 36 | 3 | 14% | 2 |
| Tropomyosin 2 (beta) | Q5TCU3_HUMAN | 33 | 3 | 11% | 2 |
| Electron transfer flavoprotein subunit beta | ETFB_HUMAN | 28 | 12 | 42% | 2 |
| Guanine nucleotide-binding protein subunit beta-2-like 1 | GBLP_HUMAN | 35 | 10 | 50% | 2 |
| Coiled-coil-helix-coiled-coil-helix domain-containing protein 3 | C9JRZ6_HUMAN | 27 | 9 | 32% | 2 |
| Calcyclin-binding prtein | CYBP_HUMAN | 26 | 9 | 55% | 2 |
| Glyceraldehyde-3-phosphate dehydrogenase | E7EUT4_HUMAN | 32 | 8 | 43% | 2 |
| Isoform 2 of Triosephosphate isomerase | TPIS_HUMAN | 27 | 8 | 44% | 2 |
| Protein NipSnap homolog 3A | NPS3A_HUMAN | 28 | 7 | 46% | 2 |
| Proteasome subunit alpha type-7 | PSA7_HUMAN | 28 | 7 | 42% | 2 |
| Serine/arginine-rich-splicing factor 1 | J3KTL2_HUMAN | 28 | 7 | 31% | 2 |
| Sepiapterin reductase | SPRE_HUMAN | 28 | 6 | 32% | 2 |
| 14-3-3 protein zeta/delta | 14332_HUMAN | 28 | 6 | 32% | 2/2L |
| Adenylate kinase 2, mitochondrial | F8W1A4_HUMAN | 26 | 5 | 24% | 2 |
| Carbonyl reductase [NADPH] | CBR1_HUMAN | 30 | 5 | 22% | 2 |
| Four and a half LIM domain 1 | Q5JX18_HUMAN | 29 | 5 | 25% | 2 |
| Heterogeneous nuclear ribonucleoprotein A3 | E7EWI9_HUMAN | 34 | 5 | 19% | 2 |
| Ras suppressoe protein 1 | RSUI_HUMAN | 32 | 4 | 19% | 2 |
| Heterogeneous nuclear ribonucleoprotein A1 | F8VRQ1_HUMAN | 33 | 4 | 15% | 2 |
| Voltage-dependent anion-selective channel protein 3 | VDAC3_HUMAN | 31 | 4 | 23% | 2 |
| Isoform 2 of cathepsin S | CAT_HUMAN | 32 | 4 | 19% | 2 |
| Carbonic anhydrase 1 | CAH1_HUMAN | 29 | 3 | 21% | 2 |
| 3-ketoacyl-CoA thiolase, peroxisomal | H7C131_HUMAN | 30 | 3 | 22% | 2 |
| Biglycan | A6NLG9_HUMAN | 35 | 3 | 11% | 2 |
| Complement C1q subcomponent subunit B | C1QB_HUMAN | 27 | 3 | 15% | 2 |
| Dihydrolipoyl dehydrogenase mitochondrial | DLDH_HUMAN | 54 | 3 | 7% | 2L |
| Leukocyte elastase inhibitor | ILEU_HUMAN | 43 | 19 | 33% | 2L |
| Complement factor H-related protein 1 | FHR1_HUMAN | 38 | 7 | 22% | 2L |
| DnaJ hololog subfamily B member 11 | DJB11_HUMAN | 41 | 7 | 24% | 2L |
| Macrophage-capping protein | B4DU58_HUMAN | 36 | 4 | 13% | 2L |
| Annexin A5 | ANXA5_HUMAN | 36 | 2 | 8% | 2L |
| Isoform short of 14-3-3 protein beta | 1433B_HUMAN | 28 | 2 | 9% | 2L |
*1: from colon cancer tissue of patient 1, male, colon cancer, stage II. 2: from colon cancer tissue of patient 2, female, colon cancer, stage IV; 2L: from liver metastatic tissue of patient 2.