Figure 4. Bioenergetic analysis of HGSC and normal FTSEC cell lines.

(A-E) Oxygen Consumption Rate (OCR) (A, C, E) and Extracellular Acidification Rate (ECAR) (B & D) measurements were obtained using an extracellular flux analyzer (Seahorse Bioscience). Basal OCR (A) and ECAR (B) rates were obtained prior to addition of Oligomycin A to derive Spare Glycolytic Capacity (D) and ATP-coupled OCR (E), and FCCP to uncouple mitochondria for maximal OCR. (C) Spare respiratory capacity was calculated by taking the difference between the maximal OCR and basal OCR. Percentages are relative to maximal respiration. (D) Spare Glycolytic Capacity was derived by taking the difference between maximal ECAR and basal ECAR. Percentages are relative to maximal ECAR. (E) Non-mitochondrial respiration was calculated as the residual OCR after treatment with Rotenone/myxothiazol that inhibits Complex I. Uncoupled mitochondrial respiration was calculated as the difference between OCR following Oligomycin A treatment and OCR following Rotenone/myxothiazol treatment. All three values are shown as percentages relative to baseline OCR.