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Nuclear marker DAPI was used to ensure that protein expression was detected only in areas with tumor cells (a) and not necrotic areas (b). Left to right, 40X montage of paraffin sections of 3 hour CA4P treated subcutaneous xenograft tumors stained with anti-PGRMC1 antibody (red), DAPI for nuclei (blue), and merged image (red and blue). Scale bar, 1 mm.
