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. 2017 Dec 23;9(3):4161–4172. doi: 10.18632/oncotarget.23647

Figure 3. Ophiopogonin B-mediated enhancement of TRAIL-initiated apoptosis is enclosed by attenuation of autophagy.

Figure 3

Cells were pre-incubated with the indicated Ophiopogonin B (10 μM) doses for 12 h and exposed to TRAIL protein for an additional 2 h. Additional cells were also pre-incubated with autophagy inhibitor chloroquine for 1 h followed by Ophiopogonin B treatment. (A) Cell morphology photographed using light microscope (×100); (B) Cell viability was measured with crystal violet assay; (C) Bar graph indicating average density of crystal violet; (D) Cell viability was assessed with trypan blue dye exclusion assays. **p < 0.01:represent significant differences between control and each treatment group; OP-B: Ophiopogonin B; TRAIL: Tumor necrosis factor (TNF)-related apoptosis-inducing ligand; CQ: Chloroquine.