Figure 4. Paraquat induces the expression of neuronal markers in NT2 cells.

(A) Cells were treated with paraquat for different durations. qPCR was performed to measure the time-dependent expression of neuronal markers. (B) Cells were treated with indicated concentrations of paraquat for two days. The dose-dependent expression of neuronal transcription factors (Pax6 and NeuroD1) and Cyp26a1 was quantified by qPCR. (C) Cells were treated with H₂O₂ at different concentrations for two days and analysed by qPCR for the transcript levels of neuronal marker genes. Bar: mean ± SD; ^*p < 0.05, ^**p < 0.01 by Student t-test.