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. 2017 Dec 30;9(3):4223–4238. doi: 10.18632/oncotarget.23786

Figure 7. Antioxidant signaling is activated by ROS and is involved in neuronal gene expression in NT2 cells.

Figure 7

(A) Cells were treated with the increasing concentrations of paraquat (PQ) for two days. The expression of the key redox signaling genes, Nrf2 and Keap1, was measured by qPCR. (B) Cells were treated with paraquat alone (left panel) or co-treated with paraquat and GSH (right panel). The expression of the antioxidant Prdm16 mRNA level was examined by qPCR. (C) Cells were transfected with siRNA targeting Nrf2 (siNrf2) and then treated with paraquat for two days. The expression of neural progenitor marker (Nestin) and neuronal markers (Grfa1 and Hoxa1) was monitored by qPCR. Bar: mean ± SD; *p < 0.05, **p < 0.01.