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. 2017 Aug 26;49(1):128–137. doi: 10.1016/j.bjm.2017.03.014

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© 2017 Published by Elsevier Editora Ltda. on behalf of Sociedade Brasileira de Microbiologia.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 1 — The sensitivity (A–D) of the LAMP or PCR for the detection of Y. pestis using serial dilutions of extracted DNA, and the specificity (E–H) of the LAMP or PCR for the detection of Y. pestis using 20 ng DNA from each bacterium of the genus Yersinia. (A) Detection of LAMP products with a real-time turbidity meter. 1–8: 20 ng–0.002 pg; 10: negative control. (B) Visualization of LAMP products stained with calcein and inspected under natural light. Tube 1–9: 20 ng–0.0002 pg; 10: negative control. (C) Detection of LAMP products with agarose gel electrophoresis. Lane 1: marker; 2–10: 20 ng–0.0002 pg; 11: negative control. (D) Electrophoretic analysis of PCR products with agarose gel. Lane 1: marker; 2–10: 20 ng–0.0002 pg; 11: negative control; 12: marker. (E) Detection of the LAMP products with a real-time turbidity meter. 1: Y. pestis; 2: Y. pseudotuberculosis; 3: Y. enterocolitica; 4: Y. frederiksenii; 5: Y. intermedia; 6: Y. kristensenii; 7: Y. bercovieri; 8: Y. mollaretii; 9: Y. rohdei; 10: Y. ruckeri; 11: Y. aldovae; 12: negative control. (F) Visualization of LAMP products stained with calcein and inspected under natural light. Tube 1: Y. pestis; 2: Y. pseudotuberculosis; 3: Y. enterocolitica; 4: Y. frederiksenii; 5: Y. intermedia; 6: Y. kristensenii; 7: Y. bercovieri; 8: Y. mollaretii; 9: Y. rohdei; 10: Y. ruckeri; 11: Y. aldovae; 12: negative control. (G) Detection of LAMP products with agarose gel electrophoresis. Lane 1: marker; 2: Y. pestis; 3: Y. pseudotuberculosis; 4: Y. enterocolitica; 5: Y. frederiksenii; 6: Y. intermedia; 7: Y. kristensenii; 8: Y. bercovieri; 9: Y. mollaretii; 10: Y. rohdei; 11: Y. ruckeri; 12: Y. aldovae; 13: negative control. (H) Electrophoretic analysis of PCR products with 1.5% agarose gel. Lane 1: marker; 2: Y. pestis; 3: Y. pseudotuberculosis; 4: Y. enterocolitica; 5: Y. frederiksenii; 6: Y. intermedia; 7: Y. kristensenii; 8: Y. bercovieri; 9: Y. mollaretii; 10: Y. rohdei; 11: Y. ruckeri; 12: Y. aldovae; 13: negative control; 14: marker.