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. Author manuscript; available in PMC: 2019 Jan 30.
Published in final edited form as: Lab Chip. 2018 Jan 30;18(3):496–504. doi: 10.1039/c7lc01052d

Figure 4.

Figure 4

Effect of aluminium mold polishing on surface roughness of COC microwells and on clarity of phase contrast microscopy images of cells grown in COC microwells. The results indicate that the highest level of polishing (SPI_A2) is required for clear phase contrast images. Microwells corresponding to the data presented in this figure are shown in zone 2 of the test device schematic (Figure 1C), with the same color-coding to represent the level of polishing applied to the metal mold. Abbreviations “PM_F0” though “SPI_A2” correspond to the polishing level options offered by Proto Labs® (see further explanation in Results section). (A) Top row: Optical microscopy images of COC microwells without cells imaged using a 3D laser scanning confocal microscope. Bottom row: Phase contrast microscopy images (10× magnification) of prostate epithelial cells (BHPrE1) grown on COC microwells; original images are included in Figure S9. Images are representative of two replicate microwells from one device. (B) Surface roughness (root mean squared (RMS) height) of COC surfaces measured using 3D laser scanning confocal microscopy. The bars indicate the mean of two replicate microwells from one device (data points from two replicates are superimposed on the bars).