PCR assay with C. burnetii performed with primers of first PCR as described in the materials and methods. Lanes: 1, negative control; 2, 100 bp DNA ladder; 3, C. burnetii suspension 1× [21.4 μg/mL]; 4, C. burnetii suspension 10×; 5, C. burnetii suspension 102×; 6, C. burnetii suspension 103×; 7, C. burnetii suspension 104×; 8, C. burnetii suspension 105×; 9, C. burnetii suspension 106×; 10, C. burnetii suspension 107×; 11, C. burnetii suspension 108×; 12, C. burnetii suspension 109×; 13, C. burnetii suspension 1010×; 14, C. burnetii suspension 1011×; 15, C. burnetii suspension 1012×; and 16, C. burnetii suspension 1013×.