Table 1.
Detection of Coxiella infection by PCR of primers (trans 1/2) and nested PCR (+N3/N4) among human, domestic animals, rodents, and tick samples collected in Rio de Janeiro between 2008 and 2015.
| Samples | PCR positivea (No. tested) | Nested PCR positiveb (No. tested) | Source location |
|---|---|---|---|
| Patients | |||
| Serum | 0 (24) | 0 (24) | |
| Clot | 2 (24) | 2 (24) | Itaboraí, RJ |
| Sheep | |||
| Serum | 0 (18) | 0 (18) | |
| Clot | 0 (18) | 0 (18) | |
| Tissue | 1 (1) | 1 (1) | Itaboraí, RJ |
| Dogs | |||
| Serum | 1 (19) | 1 (19) | |
| Clot | 0 (19) | 0 (19) | |
| EDTA blood | 1 (8) | 1 (8) | Itaboraí, RJ |
| Vaginal swabs | 0 (5) | 0 (5) | |
| Fecal swabs | 0 (8) | 0 (8) | |
| Goats | |||
| Serum | 0 (10) | 0 (10) | |
| Clot | 0 (10) | 0 (10) | Itaboraí, RJ |
| Milk | 6 (7) | 6 (7) | |
| Vaginal swabs | 0 (8) | 0 (8) | |
| Fecal swabs | 0 (3) | 0 (3) | |
| Cats | |||
| Serum | 0 (13) | 0 (13) | |
| Clot | 0 (13) | 0 (13) | |
| Fecal swabs | 1 (7) | 1 (7) | Itaboraí, RJ |
| Rodents | |||
| Spleen tissue | 0 (74) | 5 (74) | Piraí/Valença, RJ |
| Ticks | 0 (180) | 2 (180) | Itaboraí, RJ |
a
Primers target the gene IS1111 transposase elements trans 1/trans 2.
b
Primers target the gene IS1111 transposase elements of nested N3+/N4−.