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MRM ion-chromatograms of amiloride, amiloride-15N3, hydrochlorothiazide and hydrochlorothiazide-13C, d2 in (A) double blank plasma, (B) blank plasma spiked with IS, (C) at LLOQ concentration of analyte with IS, and (D) real subject sample at Cmax after oral administration of 5 mg amiloride +50 mg hydrochlorothiazide tablet formulation.
