Table 2.
Matrix factors for ergocalciferol and ergocalciferol-d6.
| QC level (ng/mL) | Ergocalciferol |
Ergocalciferol-d6 |
IS-normalized matrix factor | ||||
|---|---|---|---|---|---|---|---|
| Mean area response |
Matrix factor | Mean area response |
Matrix factor | ||||
| Post-extraction spiking | Spiking in mobile phase | Post-extraction spiking | Spiking in mobile phase | ||||
| HQC (80.0) | 7006545 | 6502566 | 1.08 | 493724 | 472568 | 1.04 | 1.04 |
| MQC-1 (40.0) | 3578144 | 3295690 | 1.09 | 483473 | 465896 | 1.04 | 1.05 |
| MQC-2 (3.00) | 287385 | 269564 | 1.07 | 493898 | 478521 | 1.03 | 1.04 |
| LQC (0.300) | 29514 | 27546 | 1.07 | 489605 | 479624 | 1.02 | 1.05 |
HQC: high quality control; MQC: medium quality control; LQC: low quality control.
Matrix factor: post-extraction spiking/spiking in mobile phase.