Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Activity of rTf_MurK is increased in the presence of magnesium ions. Product yield (MurNAc-6P) increased more than 10-fold (red) when MgCl2 was added (10 mM final concentration) to the Tf_MurK reaction mixture compared to the reaction without MgCl2 (black). Shown are the extracted ion chromatograms (EICs) for MurNAc-6P [(M-H)- = 372.072 m/z] eluting at a retention time of 17 min on ZIC-HILIC column.
